Biotechnicas

Important Topics Of Animal Cell Culture Characteristics Of Animal Cells In Culture Primary Culture Secondary Culture Difference Between Adherent Cells And Suspension Cells Parental Tissues Need For Subculturing  Types Of Cell Lines Physical Environment For Maintaining Animal Cell Lines Temperature pH Osmolality Nutrient Media Special Equipments LAF Hood CO 2 Incubator Inverted Microscope Characteristics Of Cell Lines Storage And Revival Of Cell Lines Method And Gene Delivery Into Animal Cells Transformation Microinjection Electroporation Lipofication Scale Up Roller Bottle Microcarrier Beads Spinner Culture Application Of Animal Cell Culture Tissue Plasminogen Activator Monoclonal Antibody Production (MAb) Difference Between Monoclonal And Polyclonal Antibody Hybridoma Technology Application Of MAb's Diagnostics Therapeutics Herceptin Stem Cell Technology Features Of Embryonic Stem Cells Gene Knockouts Tissue Engineering Xenograft Here are the handmade notes of 'ANIMAL CELL CUL

Important Topics Of Plant Cell Culture & Application Steps In Plant Tissue Culture Nutrient Media For Plant Tissue Culture Types Of Cultures Organ Culture Explant Culture Callus Culture Cell Suspension Culture Protoplast Culture Somatic hybridisation Zygotic Hybridisation Cybridisation Plant Regeneration Pathways Direct Organogenesis Somatic Embryogenesis Micropropagation Meristem Culture Artificial Seeds Embryo Rescue Triploid Culture + Application Primary And Secondary Metabolites(Uses) Somaclonal Variation Invitro Plant Conservation Genetic Engineering Of Plants Physical/ Vectorless Method Vector Mediated Method Agrobacterium Analysis Of Transgene Golden Rice Edible Vaccine Biodegradable Plastic Molecular Farming Biosafety Issues In Plant Genetic Engineering

  Important Topics Of Microbial Cell Culture & Application Media Culture techniques Sterilization Techniques Lab Scale Industrial Scale Types Of Cultures Batch Culture Fed-Batch Culture Continuous Culture Chemostat Turbidostat Measurement Of Microbial Growth Measurement Of Dry Weight Measuring Absorbance In Spectrophotometre Wet Weight Measurement ATP Measurement Viable Plate Count Coulter Counter Method Growth Kinetics/Specific Growth Rate Doubling Time/Generation Time Growth Kinetics With Respect To Biomass Scale Up Of Microbial Process Culture/Stain Preservation Techniques Storage On Agar Storage In Liquid N 2 Freeze Drying In Vaccum Culture Collection Centres Application Of Microbial Culture Biosafety Issues Strain Isolation, Improvement & Preservation Mutation By Genetic Engineering Problem In Expression Of Eukaryotic Gene With Prokaryotic Host Metagenomics

  Important Topics Of Genomics, Proteomics & Bioinformatics Genomics Expressed Sequence Tags Structural And Functional Genomics Need To Sequence The Complete Genome (Human Genome Project) Direct Sequencing Random Shotgun Sequencing Gene Prediction And Counting Surprises Revealed By Human Genome Project Insilico Methods Are Not Reliable No Simple Correlation Between No. Of Gene And Genome Single Nucleotide Polymorphism Functional Genomics Nick Translation Fluoroscence In-situ Hybridisation Microarray Technology Proteomics Bioinformaics Major Databases Resources Available With NCBI Database Retrieval Tools ENTREZ Taxonomy Browser Locus Link BLAST Principle  Difference Between Homologous And Paralogous  Analysis Using Bioinformatics Curator

 Important Topics Of Protein Structure & Engineering Structure Of Proteins No-Covalent Interaction In Proteins- Ionic Interactions Hydrogen Bonding Van-der-waals Interaction Hydrophobic Interaction Structure-Function Relationship In Proteins- Chymotrypsin Zymogen Insitu Activation Chymotrypsin Mechanism Example Of Reactive Serine Meachanism Peptide Mapping / Protein Fingerprinting 2-D Gel Electrophoresis Protein Purification- GRAS Organisms Plants Animals Molecular Farming Factors Upon Which Purification Scheme Depends Upon Microbial Source- Intracellular Proteins Extracellular Proteins Specific Activity- Ion-Exchange Chromatography Affinity Chromatography Site Exclusion Chromatography Protein Based Products Mass Spectrometry Matrix Assisted Laser Ionisation Desorption Ionisation (MALDI) Protein Based Products Nutraceutical Proteins Designing Proteins Biological Value Protein Efficiency Ratio BCAA Novel Vaccines Advantages Of Protein Subunit Vectors

Important Topics Of Recombinant DNA Technology- Basic Steps In Recombinant DNA Techonology Tools In Recombinant DNA Technology- Enzymes Vectors Restricted Endonucleases Restriction Endonucleases Type II R.E Restriction Enzymes Alu I BamH I EcoR I Role Of R.E in bacteria Nomenclature Of R.E Type II R.E Sticky End Blunt End Polymerase Chain Reaction Southern Hybridisation DNA Library Genomic DNA Library cDNA Library DNA Sequencing Site Directed Mutagenesis