Protein Structure And Engineering

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 Important Topics Of Protein Structure & Engineering

  • Structure Of Proteins
  • No-Covalent Interaction In Proteins-
  1. Ionic Interactions
  2. Hydrogen Bonding
  3. Van-der-waals Interaction
  4. Hydrophobic Interaction
  • Structure-Function Relationship In Proteins-
  1. Chymotrypsin
  2. Zymogen
  3. Insitu Activation
  4. Chymotrypsin Mechanism
  • Example Of Reactive Serine Meachanism
  • Peptide Mapping / Protein Fingerprinting
  • 2-D Gel Electrophoresis
  • Protein Purification-
  1. GRAS Organisms
  2. Plants
  3. Animals
  4. Molecular Farming
  • Factors Upon Which Purification Scheme Depends Upon
  • Microbial Source-
  1. Intracellular Proteins
  2. Extracellular Proteins
  • Specific Activity-
  1. Ion-Exchange Chromatography
  2. Affinity Chromatography
  3. Site Exclusion Chromatography
  4. Protein Based Products
  5. Mass Spectrometry
  6. Matrix Assisted Laser Ionisation Desorption Ionisation (MALDI)
  • Protein Based Products
  • Nutraceutical Proteins
  • Designing Proteins
  • Biological Value
  • Protein Efficiency Ratio
  • BCAA
  • Novel Vaccines
  • Advantages Of Protein Subunit Vectors


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