Animal Cell Culture

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Important Topics Of Animal Cell Culture

  • Characteristics Of Animal Cells In Culture
  • Primary Culture
  • Secondary Culture
  • Difference Between Adherent Cells And Suspension Cells
  • Parental Tissues
  • Need For Subculturing 
  • Types Of Cell Lines
  • Physical Environment For Maintaining Animal Cell Lines
  1. Temperature
  2. pH
  3. Osmolality
  • Nutrient Media
  • Special Equipments
  1. LAF Hood
  2. CO2 Incubator
  3. Inverted Microscope
  • Characteristics Of Cell Lines
  • Storage And Revival Of Cell Lines
  • Method And Gene Delivery Into Animal Cells
  1. Transformation
  2. Microinjection
  3. Electroporation
  4. Lipofication
  • Scale Up
  1. Roller Bottle
  2. Microcarrier Beads
  3. Spinner Culture
  • Application Of Animal Cell Culture
  • Tissue Plasminogen Activator
  • Monoclonal Antibody Production (MAb)
  • Difference Between Monoclonal And Polyclonal Antibody
  • Hybridoma Technology
  • Application Of MAb's
  1. Diagnostics
  2. Therapeutics
  3. Herceptin
  • Stem Cell Technology
  • Features Of Embryonic Stem Cells
  • Gene Knockouts
  • Tissue Engineering
  • Xenograft

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